Effect of Crude Sulphated Polysaccharide from Marine Brown Algae in Tpa Induced Inflammation on Poly Morphonuclear Leukocytes

Objective: The present study aims to evaluate the anti-inflammatory activity of crude sulphated polysaccharide from marine brown algae sargassum ilicifolium Methods: The present study involves the investigation of crude sulphated polysaccharide from marine brown algae sargassum ilicifolium for antiinflammatory activity by TPA [12OTechanoyl 13 – Myristate] induced inflammation in Polymorphonuclear leukocytes [PMNL]. T.h.e cell viability by Trypan blue dye exclusion assay, MTT assay, release of Cathepsin D, Nitrite, TNF-α levels was assessed. Diclofenac was used as the standard drug for comparison. Results: Crude sulphated polysaccharide were isolated from brown seaweed sargassum ilicifolium. Crude sulphated polysaccharide showed the significant increase in the number of viable PMNL cells. It has further reduced the release of Cathepsin D, Nitrite and TNF-α. The levels of which are increased during inflammatory conditions. Conclusion: Therefore our studies support the isolation and the use of sulphated polysaccharide in treating inflammation and rheumatism.